Fig. 4.
Coactivator recruit assay using recombinant human steroid receptor coactivator 1 (hSRC1) and 35S-methanoine labeled FXR and RARα. Purified GST-hSRC1 (fragment) fusion protein on glutathione beads were used to pull down 35S-FXR and 35S-RARα with or without addition of CDCA or atRA. atRA, all-trans retinoic acid; CDCA, chenodeoxycholic acid; FXR, farnesoid X receptor; GST, glutathione S-transferase; RARα, retinoic acid receptor α; SRC1, steroid receptor coactivator 1.