TABLE 1.
Partial protein content of RBL-2H3wt exosomes
| Protein Category | Accession Number | Protein Type | Observations |
|---|---|---|---|
| A) Exosome markers | Q62351 | Transferrin receptor | |
| P41731 | CD63 antigen | Tetraspanin | |
| P40237 | CD82 antigen | Tetraspanin | |
| P35762 | CD81 antigen | Tetraspanin | |
| P11499 | Heat-shock protein 90-β | ||
| P63017 | Heat-shock cognate 70 kDa (Hsc70) | Interacts with phospholipase A2 (iPLA2) | |
| B) Lipid-related proteins | Q8K4S1 | Phosphoinositide-specific phospholipase C ϵ | Involved in phosphoinositide signaling |
| Q9JJ00 | Phospholipid scramblase | Mix phospholipids between membrane leaflets | |
| Q05816 | Fatty acid binding protein (E-FABP) | Free fatty acid transporter | |
| Q5SRA8 | Prostaglandin F2 receptor negative regulator | Interacts with CD81 | |
| C) Phospholipase partners | P05064 | Fructose- bisphosphate aldolase | Interacts with phospholipase D (PLD2) |
| P63017 | Heat Shock protein 70 kDa | Interacts with phospholipase A2 (iPLA2) | |
| P67871 | Casein kinase II β subunit | Interacts with phospholipase A2 (sPLA2) | |
| D) GTP binding proteins | Heterotrimeric | ||
| P08752 | G(i)α-2 subunit | ||
| P62874 | G(i)G(s) β subunit 1 | ||
| P62880 | G(i)G(s) β subunit 2 | ||
| Monomeric GTPases | |||
| P35278 | Ras-related prorein Rab | ||
| Q9QUI0 | Transforming protein RhoA | Interacts and activates phospholipase D (PLD2) | |
| P63835 | Ras-related protein Rap-1A | ||
| Q61411 | Transforming protein P21/H-Ras-1 (c-H-Ras) | ||
| Q61820 | GTP-binding nuclear protein Ran | ||
| P62331 | ADP-ribosylation factor 6 | Interacts and activates phospholipase D (PLD2) | |
| Q8BGX0 | GTP-binding protein ARD-1 | ||
| (ADP-ribosylation factor domain protein 1) |
Only proteins related to exosome markers, lipid metabolism, and G proteins are reported. The overall analysis identified 382 different proteins. Observations are detailed in the text.