TABLE 2.
Mouse hepatic cardiolipin molecular species distribution analyzed by MDMS-SL and dynamic simulation
| Carbon: Double Bond | Major Species | [CL-2H]2- | Mean Mass Content | Mean Distribution of Species (%) | Distribution Generated by Dynamic Simulation (%) |
|---|---|---|---|---|---|
| 68:6 | 0.26 | ||||
| 68:5 | 0.15 | ||||
| 68:4 | 0.03 | ||||
| 70:8 | 0.01 | ||||
| 70:7 | 18:2-18:2-18:2-16:1 | 710.97 | 1.49 | 5.99 | 5.52 |
| 70:6 | 18:2-18:2-18:1-16:1 | 711.98 | 0.65 | 2.60 | 3.04 |
| 18:2-18:2-18:2-16:0 | |||||
| 70:5 | 18:2-18:1-18:1-16:1 | 712.99 | 0.15 | 0.62 | 0.66 |
| 18:2-18:2-18:1-16:0 | |||||
| 70:4 | 18:2-18:1-18:1-16:0 | 713.99 | 0.19 | 0.78 | 0.07 |
| 72:9 | 0.17 | ||||
| 72:8 | 18:2-18:2-18:2-18:2 | 723.98 | 11.26 | 45.30 | 44.16 |
| 72:7 | 18:2-18:2-18:2-18:1 | 724.99 | 5.57 | 22.39 | 23.48 |
| 72:6 | 18:2-18:2-18:1-18:1 | 725.99 | 1.41 | 5.68 | 4.99 |
| 72:5 | 18:2-18:2-18:1-18:0 | 727.00 | 0.06 | 0.26 | 0.54 |
| 18:2-18:1-18:1-18:1 | |||||
| 72:4 | 0.03 | ||||
| 74:11 | 18:2-18:2-16:1-22:6 | 734.97 | 0.03 | 0.10 | 0.12 |
| 74:10 | 18:2-18:2-18:2-20:4 | 735.98 | 0.22 | 0.88 | 0.71 |
| 18:2-18:1-16:1-22:6 | |||||
| 18:2-18:2-16:0-22:6 | |||||
| 74:9 | 18:2-18:2-18:2-20:3 | 736.99 | 1.34 | 5.39 | 4.74 |
| 74:8 | 18:2-18:2-18:2-20:2 | 737.99 | 1.42 | 5.70 | 5.66 |
| 74:7 | 18:1-18:2-18:2-20:2 | 739.00 | 0.47 | 1.87 | 1.91 |
| 74:6 | 18:1-18:2-18:2-20:1 | 740.01 | 0.11 | 0.43 | 0.29 |
| 18:1-18:1-18:2-20:2 | |||||
| 74:5 | 0.02 | ||||
| 76:12 | 18:2-18:2-18:2-22:6 | 747.98 | 0.32 | 1.29 | 1.25 |
| 76:11 | 18:2-18:2-18:2-22:5 | 748.99 | 0.17 | 0.70 | 0.73 |
| 18:1-18:2-18:2-22:6 | |||||
| 76:10 | 0.38 | ||||
| 76:9 | 0.37 | ||||
| 76:8 | 0.22 | ||||
| 76:7 | 0.05 | ||||
| 78:14 | 0.01 | ||||
| 78:13 | 0.10 | ||||
| 78:12 | 0.12 | ||||
| 78:11 | 0.05 | ||||
| 78:10 | 0.02 | ||||
| 80:16 | 0.01 |
Mouse hepatic mitochondrial lipids were extracted by a modified Bligh and Dyer procedure as described in the section of “Materials and Methods.” The cardiolipin molecular species in the lipid extracts were identified and quantified as previously described (15). The mass content and distribution of cardiolipin species represent the mean of four separately prepared mitochondria from different animals. The distribution of cardiolipin molecular species determined by the dynamic simulation represents the one frame output 0.1α 250 iterations for liver cardiolipin. This data was obtained using the MS output function. Values < 0.01 percent are not displayed but can be found in supplementary tables. CL, cardiolipin.