TABLE 1.
Comparison of the kinetic parameters of AEA transport obtained by the two procedures in human HaCaT cells and SH-SY5Y cells
| Import |
Export |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Km (μM) |
Vmax [pmol/(min·mg)] |
t1/2 (min) |
t1/2 (min) |
|||||
| Cell Line | Plastic | Glass | Plastic | Glass | Plastic | Glass | Plastic | Glass |
| HaCaT | 0.47 ± 0.15 | 0.27 ± 0.05** | 125 ± 15 | 130 ± 1 | 5.1 ± 2.7 | 2.5 ± 0.7* | 2.4 ± 0.8 | |
| SH-SY5Y | 0.49 ± 0.23 | 0.46 ± 0.12 | 41.5 ± 5.2 | 16.2 ± 1.9*** | 1.1 ± 0.2 | |||
Data are means ± SD values, n = 9 for each value. * Indicates P < 0.05 versus plastic control; ** indicates P < 0.01 versus plastic control; *** indicates P < 0.001 versus plastic control.