Fig. 3.

Transacylase activity in human plasma. A: The reaction mixture contained 48 mM sodium citrate (pH 4.5), liposomes (130 µM phospholipid), and 1.6% of human plasma in 500 µl of total volume. The liposomes consisted of DOPG/NAS (3:1, molar ratio). The reaction was initiated by adding 8 µl of human plasma and kept for 30, 60, and 90 min at 37°C. The reaction product was determined and quantified as described in Fig. 2 and plotted against incubation time (B). C: Different concentrations of plasma were incubated with DOPG/NAS liposomes for 90 min at 37°C in 500 µl of 48 mM Na-citrate (pH 4.5). D: The 1-O-acyl-NAS formed after 90 min incubation was plotted against the plasma concentration in the reaction mixture.