Fig. 4.

The absence of transacylase activity in LPLA2-knockout mouse plasma. The reaction mixture contained 48 mM sodium citrate (pH 4.5), 10 µg/ml BSA, liposomes (130 µM phospholipid), 4% mouse plasma, or 4% LPLA2-knockout mouse plasma in 500 µl of total volume. Liposomes consisting of DOPG/NAS (3:1, molar ratio) were incubated with the mouse plasma for 90 min at 37°C. The reaction products were extracted and separated by an HPTLC plate using a solvent system consisting of chloroform/acetic acid (9:1, v/v).