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. Author manuscript; available in PMC: 2011 Jul 13.
Published in final edited form as: Biochemistry. 2010 Jul 13;49(27):5662–5670. doi: 10.1021/bi100710a

Figure 4.

Figure 4

Spectroelectrochemical potentiometric redox titrations of full-length GLB-6. The intensity of the α-band maxima, A560, of ferrous GLB-6 minus A560 of ferric GLB-6 is normalized and plotted versus the ambient potential (inset). The data were analyzed for the best fit to Nernst equations with n = 1 (inset, dotted line). The measured redox potential versus SHE, E0’, is −193 ± 2 mV, which is an average of three experiments. (Buffer: 100 mM potassium phosphate, 150 mM NaCl, pH 7.0, Temperature = 25 °C).