Figure 8.

Oocytes require AR expression for androgen- but not progestin-mediated maturation. Denuded oocytes isolated from 21-d-old oocyte-specific AR −/− and +/− mice (n = 2 animals per genotype) were treated with DHT (100 nm), R5020 (Promegestone, 250 nm), or ethanol (0.1%). Maturation (GVBD) of denuded oocytes was scored at 4 h and 8 h after treatment. Twenty-five oocytes per animal for each treatment were used for the experiment, and data are represented as the combined percent of oocytes that had undergone GVBD from two separate experiments. Data were analyzed using χ² test and P ≤ 0.01 for DHT-treated oocytes isolated from oocyte-specific AR −/− vs. +/− mice.