Skip to main content
. 2010 Jun 2;24(7):1423–1433. doi: 10.1210/me.2009-0463

Figure 5.

Figure 5

ATF3 contributes to the up-regulation of insulin gene expression in primary islets. A, Primary islets isolated from WT or KO mice were allowed to recover for 72 h in 5.5 mm glucose before 33 mm glucose treatment for the indicated time and analyzed by immunoblot for ATF3 or actin. B, Primary islets as described in panel A were analyzed for mature insulin 1 by qRT-PCR and for ATF3 mRNA as a control. Shown are the averages from three experiments. C, Primary islets as described in panel A were analyzed for preinsulin 2 (pre-Ins2) RNA by RT-PCR and for ATF3 and actin mRNAs as controls. Shown are two representatives of a total of five experiments. D, Pre-Ins2 signals as obtained in panel C from five experiments were quantified. Presented are means ± sem. Black bars represent WT; white bars represent KO. *, P < 0.05; **, P < 0.006 KO vs. WT with the corresponding glucose treatment. Exp., Experiment.