Figure 1.

PPAR and RXR expression by HEC-1A and HES cells. Total RNA was isolated from human liver (L), HES (S), and HEC-1A (C) cells, and amplicons for PPARα, PPARβ, PPARγ, RXRα, RXRβ, and RXRγ were generated by RT-PCR as described in Materials and Methods. A molecular weight ladder is indicated to the left of the panels. Note that all three PPAR transcripts, as well as those for RXRα and RXRβ, are expressed in both cell lines. However, RXRγ transcript is barely detectable in the cell lines. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.