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. 2010 Apr 21;151(7):3420–3431. doi: 10.1210/en.2009-1359

Figure 6.

Figure 6

Decreased ERK(1/2) phosphorylation in Sulf1-transfected UGS tissues. UGS tissues were cultured with testosterone for 48 h after transfection with the Sulf1- or βgal-expressing plasmids. A, Real-time RT-PCR analysis of mRNA extracts from transfected UGSs indicate that Sulf1 expression was significantly increased relative to control (βgal-transfected) UGS tissues. *, P = 0.0002 by unpaired t test. B, Western blot analysis of UGS protein extracts confirmed increased SULF1 protein content in the Sulf1-transfected UGSs relative to control (βgal) transfected tissues and phosphorylated FGFR (nonspecific for FGFR paralogs) is decreased in Sulf1-transfected UGS tissues. C, Phosphorylated ERK(1/2) protein is decreased in Sulf1-transfected tissues relative to control UGSs. Total ERK(1/2) protein content was used as a loading control in each blot.