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Effect of glucose on binding of AP-1 regulatory elements in predicted PRR promoter. Panels A–D, In vivo mapping of AP-1 by ChIP. Panel E, EMSA and competitive EMSA. Oligo, AP-1 probes to AP-1 binding elements (Panels C–F as shown in Fig. 1). NE, Nuclear extract. Glucose (−), Control (5 mm d-glucose+25 mm l-glucose); glucose (+), 30 mm d-glucose. ChIP assay is based on three independent experiments and EMSA is a representative of three experiments.
