Table 1.
Application | Target element | Oligonucleotide | Sequence (5′–3′) |
---|---|---|---|
RT-PCR | PRR forward | TGGCCTATACCAGGAGATCG | |
PRR reverse | AATAGGTTGCCCACAGCAAG | ||
β-Actin forward | AGCCATGTACGTAGCCATCC | ||
β-Actin reverse | ACCCTCATAGATGGGCACAG | ||
ChIP | C | Prom-1145F | CCATTCCGAGTCACCCTCT |
Prom-1031R | TCTCATCCTCCTGTCTTGATTTT | ||
A, D | Prom-976F | AGGGATGGTATATGCGATGG | |
Prom-808R | ACCGAGTATCCGAGAATGGA | ||
E | Prom-375F | CCACGTTCTAGCCCTTTCTG | |
Prom-143R | CCGTACGAGACGGTTATCCT | ||
F, G, B | Prom-184F | GGGACGAGAATTTTGGAAAC | |
Prom-25R | AGGGAGGGGAAATCTGAGAG | ||
EMSA | A | NF-κB01 | AGTATAAGTAGAAAGTTCCAGTAGTCGACC |
B | NF-κB02 | GATGCAGAGAGAGGACATCGCCGCATCAAC | |
G | Sp1 | GTAGTGCAATCAGGGGCGGGGTTAACGCTTGCAGTGGGTCCGATGCAGAG | |
C | AP-1.1 | AACTCCATTCCGAGTCACCCTCTCGGGAAC | |
D | AP-1.2 | AACAAAAGATTGGTGTCTAATACACGGGTG | |
E | AP-1.3 | GTTCTAGCCCTTTCTGACTACGGGTACAAC | |
F | AP-1.4 | ACCGTCTCGTACGGTGAGTAGTGCAATCAG |