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. 2010 May 5;151(7):3317–3325. doi: 10.1210/en.2009-1368

Table 1.

Oligonucleotides used in RT-PCR, ChIP assay, and EMSA

Application Target element Oligonucleotide Sequence (5′–3′)
RT-PCR PRR forward TGGCCTATACCAGGAGATCG
PRR reverse AATAGGTTGCCCACAGCAAG
β-Actin forward AGCCATGTACGTAGCCATCC
β-Actin reverse ACCCTCATAGATGGGCACAG
ChIP C Prom-1145F CCATTCCGAGTCACCCTCT
Prom-1031R TCTCATCCTCCTGTCTTGATTTT
A, D Prom-976F AGGGATGGTATATGCGATGG
Prom-808R ACCGAGTATCCGAGAATGGA
E Prom-375F CCACGTTCTAGCCCTTTCTG
Prom-143R CCGTACGAGACGGTTATCCT
F, G, B Prom-184F GGGACGAGAATTTTGGAAAC
Prom-25R AGGGAGGGGAAATCTGAGAG
EMSA A NF-κB01 AGTATAAGTAGAAAGTTCCAGTAGTCGACC
B NF-κB02 GATGCAGAGAGAGGACATCGCCGCATCAAC
G Sp1 GTAGTGCAATCAGGGGCGGGGTTAACGCTTGCAGTGGGTCCGATGCAGAG
C AP-1.1 AACTCCATTCCGAGTCACCCTCTCGGGAAC
D AP-1.2 AACAAAAGATTGGTGTCTAATACACGGGTG
E AP-1.3 GTTCTAGCCCTTTCTGACTACGGGTACAAC
F AP-1.4 ACCGTCTCGTACGGTGAGTAGTGCAATCAG