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. 2010 May 12;151(7):2974–2984. doi: 10.1210/en.2010-0033

Figure 6.

Figure 6

Regulatory region for prolactin/STAT5 stimulation of 1α(OH)ase transcription and induction of 1α(OH)ase protein by prolactin. A, AOK B-50 cells were transfected with 0.3 μg mouse 1α(OH)ase promoter −1651/+22 or deletion constructs −477/+22, −233/+22, −144/+22, and −85/+22. In some wells, STAT5a expression vector (50 ng) was also transfected. After 24 h, cells were treated with vehicle or prolactin (400 ng/ml) for 24 h. Results are the mean ± sem of six to eight observations/group. B, Western blot analysis was performed using total extracts from AOK B-50 cells. Cells were transfected with STAT5a and prolactin receptor and treated with vehicle (0) or prolactin (400 ng/ml) for 3–48 h. Prolactin treatment resulted in a significant increase in 1α(OH)ase protein levels at 3, 6, 12, and 24 h after prolactin treatment (P < 0.05 compared with vehicle).