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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: Biotech Histochem. 2009 Oct;84(5):223–247. doi: 10.3109/10520290903039094

Figure 1.

Figure 1

DUI45 cells were either not fixed (0 min.) (1A-A) or fixed in 10% NBF at 4°C for 5 min. (1A–B), 6 hr. (1A–C), 24 hr. (1A–D), 48 hr. (1A–E) or 72 hr. (1A–F), washed, subjected to no antigen retrieval or to antigen retrieval, and then immunostained to detect the Ki67 antigen. Panel A demonstrates the percentage of cells stained at each intensity (1A-A to 1A–F). Panel B demonstrates the immunostaining scores calculated based on the intensity of staining and the proportion of cells staining at each intensity.