Figure 2. CD62L^low/CD4⁺ effector T cells differentiate into T_EM and T_CM in vivo.

Purified Thy1.1⁺/CD62L^low/CD4⁺ T cells were activated in vitro and 4×10⁷ cells were adoptively transferred to irradiated tumor-free Thy 1.2⁺ congenic mice. (A) Day 82 post transfer, the percentage of Thy 1.1⁺ cells in the spleen and LNs was determined. Among donor T cells, 100% were CD44^high and >95% were CD4⁺ with high expression of CD62L on 50.2% in spleen or 81.3% in LN. (B) Day 143 post transfer, Thy 1.1⁺ T cells were analyzed for CD62L expression in spleen, LNs, blood, and lung. The numbers represented the percentage of donor cells in the dot-plot quadrants. Each experiment included 2 mice.