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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: J Immunol. 2010 Apr 9;184(10):5612–5618. doi: 10.4049/jimmunol.0903549

Figure 6. Reactivated TEM and TCM CD4+ cells have similar therapeutic efficacy.

Figure 6

Thy1.1+ TCM or TEM cells isolated 276 days after adoptive transfer to tumor-free hosts were activated in vitro with anti-CD3/IL-2/IL-7. (A) The indicated number of cells initially derived from TCM or TEM was adoptively transferred to hosts bearing 3-day MCA 205 pulmonary metastases. Day 20 later, lungs were harvested and tumors were enumerated. Transfer of 5×106 TCM or TEM cells effectively eradicated pulmonary metastases (p<0.01 compared with control group). There was no significant difference between TCM vs. TEM groups at the two doses. (B) Culture-activated TCM or TEM cells were incubated alone (no stimulation), with a single-cell MCA 205 or MCA 207 tumor digest, or anti-CD3 and stained for intracellular IFN-γ. A similar percentage of TCM or TEM produced IFN-γ in response to MCA 205 stimulation (13% v.s 14%).