. Author manuscript; available in PMC: 2011 May 15.

Published in final edited form as: J Immunol. 2010 Apr 9;184(10):5612–5618. doi: 10.4049/jimmunol.0903549

Table 1.

Proliferation, phenotype and IFN-γ response of activated CD4⁺ or CD8⁺ T cells

T cell subset and cytokines	Prolif. X-fold	FACS (%)		IFN-γ (%) positive among CD4⁺ or CD8⁺ cells
		CD4⁺	CD8⁺	no-stim	MCA205	MCA207	Anti-CD3
CD4⁺, IL-7/IL-23	1,123	97	0.3	0	31	0.8	91
CD8⁺, IL-7/IL-23	2,673	0.3	96	0.2	32	0.3	97
CD4⁺, IL-2/IL-7	3,447	99	0.2	0	5	0.1	70
CD8⁺, IL-2/IL-7	12,174	0.2	95	0.2	13	0.5	93

CD62L^low T cells were isolated from tumor-draining lymph nodes and CD4⁺ or CD8⁺ subsets were additionally purified and activated with anti-CD3 mAb on day 0–2 and day 23 and were maintained in IL-2 + IL-7 or in IL-7 + IL-23. The total proliferation over the 29 day culture period was calculated. The phenotype of each culture was determined by FACS staining. The IFN-γ production in response to incubation with the MCA 205 tumor used for priming of LNs or an antigenically distinct tumor MCA 207 or anti-CD3 mAb was determined.