Table 4.

Summary of EPR and CD Titration Data for the Octarepeat, PrP(23–28, 73–91), and PrP(23–28, 57–91) (cf. Figures 5 and 6)

	octarepeat (equiv)	PrP(23–28, 73–91) (equiv)	Prp(23–28, 57–91) (equiv)
max EPR	0.92	1.90	4.24
signal intensitya
Cu2+ conc	0.83	1.88	3.83
detected by EPRb
max CD	1.06	2.53	4.37
molar ellipticityc
normalized CD	1.00	2.00	4.19
molar ellipticityd

^aEquivalents of Cu²⁺ added to achieve maximum EPR signal intensity.

^bAbsolute bound Cu²⁺ concentration detected by EPR.

^cEquivalents of Cu²⁺ added to achieve maximum CD molar ellipticity.

^dMaximum molar ellipticity scaled as integer value of octarepeat molar ellipticity when fully loaded with Cu²⁺.