Figure 5.

Taz shRNA blocks the proliferation of RPE cells in culture and nuclear expression of Tead1 and Zeb1. RPE cells were infected on day 2 in primary culture with lentiviruses expressing GFP and either Taz shRNA or a control scrambled shRNA sequence. (A) Two days after infection (day 0), GFP+ (infected) and GFP− (uninfected) RPE cells were initially counted. Three days later (day 3), the same areas were again counted. GFP+ cells were counted again after 14 days. GFP− cells were not counted because they had become too numerous. Results represent an average of five different areas. (B) After 2 weeks in culture, cells were immunostained, and nuclear localization was quantified in comparison with cytoplasmic localization of Zeb1 and Tead1.