Figure 5.

Nf2^CKO lens cells lost apical-basal polarity. (A) Staining for ZO-1 was restricted to the apical ends of WT cells in the invaginating lens vesicles. (B) In Nf2^CKO lens vesicle cells, ZO-1 was also present along the lateral (arrow) and basal (arrowheads) ends. (C) At E12.5, ZO-1 was expressed at the apical ends of epithelial and fiber cells of Nf2^WT lenses, especially near the lens equator (arrowheads). (D) In Nf2^CKO lenses, ZO-1 was expressed throughout the fiber region, especially along fiber cell lateral membranes (arrowheads). Scale bars: 20 μm (A, B); 50 μm (C, D).