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. 2010 Jul;51(7):3680–3686. doi: 10.1167/iovs.09-4575

Figure 1.

Figure 1.

(A) Marked upregulation of TNF-α, iNOS, and αA crystallin were found in WT mice with EAU retinas compared with nude mice with EAU, TLR4−/− mice with EAU, and nonimmunized controls. Upregulation of TNF-α, iNOS, and αA crystallin was also found in nude mice with EAU compared with TLR4−/− mice with EAU and nonimmunized controls. TNF-α, iNOS, and αA crystallin were all downregulated in TLR4−/− mice with EAU compared with nonimmunized WT controls. (B) TNF-α, iNOS, and αA crystallin were not upregulated in MyD88−/− mice. iNOS and αA crystallin were not elevated in TNF-α−/− mice. Similarly, there was no upregulation of αA crystallin or TNF-α in iNOS−/− mice. Gene expression of TNF-α, iNOS, and αA crystallin during early EAU in C57BL/6 (WT), B6.Cg-Foxn1nu (nude), TLR4−/−, MyD88−/−, TNF-α−/−, and iNOS−/− mice by real-time PCR analysis using gene-specific primers and normalized to GAPDH. The relative multiple of change in mRNA expression was determined using the 2-ΔΔCt method. *P < 0.05; **P < 0.001.