Figure 3.

Immunofluorescence localization of αA crystallin and iNOS in the retina. Tissues were labeled using polyclonal antibody against αA-crystallin and monoclonal anti-iNOS (primary antibodies) and against Cy-2–conjugated donkey anti-rabbit IgG and Texas Red dye–conjugated donkey anti-mouse IgG, respectively (secondary antibodies). (A) Immunostaining of nonimmunized C57BL/6 (WT) mice showed αA crystallin localized in the outer plexiform layer and the photoreceptor inner segments. Nonimmunized TLR4^−/− mice also showed very weak staining in these retinal layers. In both nonimmunized WT and TLR4^−/− mice, there was no detectable amount of iNOS staining. (B) Immunostaining of WT mice EAU day 7 after immunization revealed intense staining of iNOS and αA crystallin in the photoreceptor inner segments and outer plexiform layer; however, minimal staining was present in the inner segments and outer plexiform layer in TLR4^−/− mice with EAU.