Figure 4.

SiRNA silencing visualized at the microscopic level in the Tg CBL∕hMGFP mouse model. Irrelevant control siRNA and specific siRNA were injected intradermally into the left and right footpad, respectively. (a) Ex vivo skin sections after two weeks of treatment. Scale bars are 20 μm. (b) In vivo imaging of footpads after 18 days of treatment using the DAC microscope. Sequential time points correspond to days post-treatment. The 3-D volume dimensions are 250×80×100 μm³. (c) Quantification of GFP signal levels from image stacks taken with the DAC microscope. Each footpad at each timepoint is imaged over multiple sites. Each column corresponds to the average of the datasets, and bars indicate the standard deviation. For days 3 and 5, the difference between left and right footpad are statistically significant, while for day 39, the difference is not statistically significant.