Figure 1.

Representative enzymatic activity analyses for aldolase, pyruvate kinase and isocitrate dehydrogenase. Activity measurements were performed on 10 TM tissue samples (5 µg total protein each) derived from normal (dotted bars) or glaucomatous donors (hollow bars). Colorimetric assay was performed for determination of enzymatic activities and standard deviation from 10 individual measurements have been presented. A: Activity assay for aldolase, B: Activity assay for pyruvate kinase, and C: Activity assay measurement for isocitrate dehydrogenase. D: Activity assay for malate dehydrogenase. Each bar represents the mean±standard deviation from ten independent experimental readings (ten samples in each group) and was found significantly different from 0.0 for each activity measurement by the one-sample t-test. The asterisk indicates a p<0.05.