Fig. 1.

Large tumor suppressor gene 1 (LATS1) is coimmunoprecipitated with forkhead L2 (FOXL2). Mammalian Chinese hamster ovary (CHO) cells were transfected with an empty expression vector (−) or FLAG-FOXL2 expression construct (+). Twenty-four hours after transfection, the cells were lysed, and the lysates were immunoprecipitated with a control mouse IgG or an antibody to FLAG. The lysates (Lysate) and immunoprecipitates (IP) were analyzed by immunoblotting with FOXL2 and LATS1 antibodies. When the empty pFLAG-CMV-2 vector was used as a template, FOXL2 was not synthesized (Lysate, −), but when the pFLAG-CMV-2-FOXL2 construct was used as a template, FOXL2 was synthesized (Lysate, +). Some endogenous LATS1 expression was also detected in the lysates prior to immunoprecipitation. When these lysates were immunoprecipitated with mouse IgG, a faint band was obtained for FLAG-FOXL2 in immunoprecipitates from cells expressing FLAG-FOXL2, and no band was obtained for LATS1 (IP:mouse IgG). When the lysates were immunoprecipitated with an antibody to FLAG, endogenous LATS1 was coimmunoprecipitated in cells expressing FLAG-FOXL2 (IP:FLAG, +) but not in cells expressing the empty expression vector (IP:FLAG, −).