Fig. 4.

LATS1 gene is expressed in developing mouse ovary. Total RNA was extracted from whole ovaries of fetal [17.5 days postconception (dpc)], immature (days 13 and 23), and adult mice, reverse transcribed, PCR amplified with LATS1 primers, and analyzed by electrophoresis. Human Ovary PCR-Ready cDNA (Ambion, Austin, TX) was used as a positive control (+), and water was used as a negative control (−). The identities of the LATS1 PCR products were confirmed by sequencing. FOXL2 expression in the same cDNAs (41) is shown for comparison.