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miR-34a is expressed in normal endothelial progenitor cells (EPCs) and increases significantly following transfection with miR-34a mimic. miR-34a expression was determined by real-time PCR. The relative gene expression was normalized to U6 small nucleolar RNA, and analysis was performed by the 2−ΔΔCT method. Data were expressed as means ± SE; n = 3. *P < 0.05 vs. scramble. HUVECs, human umbilical vein endothelial cells.
