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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Gene Ther. 2009 Oct 1;17(3):315–327. doi: 10.1038/gt.2009.126

Figure 3. Calpain and caspases-7 and -3 are activated in ΔPK infected melanoma cells.

Figure 3

(a) A2058 cells were infected with ΔPK (moi = 0.5) or mock infected with PBS in the absence or presence of the calpain inhibitor PD150606 (100 μM) and cell extracts obtained at various times p.i. were immunoblotted with antibody to calpain that recognizes the inactive (p80), activated (p76) and regulatory (p28) species. Data were quantified by densitometric scanning, as described in Materials and Methods and results are expressed as the ratio of the p76/p80 and p28 densitometric units ± SD respectively. Representatives of three replicate experiments are shown (***p<0.001 vs. Mock). (b) The immunoblots in panel (a) were sequentially stripped and re-probed with antibodies to activated caspase-7, activated caspase-3, and actin. Data were quantified by densitometric scanning, as described in Materials and Methods, and results are expressed as densitometric units ± SD (**p<0.01, ***p<0.001 vs. Mock). (c) Extracts of A2058 melanoma cells infected with ΔPK (moi = 0.5) with or without z-VAD-fmk (Sigma-Aldrich, 100μM or Promega, 20μM) and cell extracts obtained at 24h p.i. were immunoblotted with antibody to activated caspase-7. Representatives of three replicate experiments are shown (***p<0.001 vs. ΔPK alone).