TABLE 3. Fold change in endometriotic lesion versus eutopic uterus: comparison between Illumina MouseRef-8 microarray and real-time RT-PCR.
3 days after induction | 29 days after induction | |||
---|---|---|---|---|
Gene | Microarray | Real time RT-PCR | Microarray | Real time RT-PCR |
Hp | 2.42a | 5.22a | 2.67a | 3.15a |
Mmp3 | 51.0a | 132a | 1.56 | 1.30 |
Mmp10 | 31.9a | 381a | 1.06 | 0.58 |
Mmp12 | 7.54a | 87.0a | 3.19a | 9.88a |
Mmp13 | 2.52a | 938a | 2.69a | 2.12 |
Timp1 | 12.6a | 18.5a | 5.01a | 3.93a |
Note: Illumina MouseRef-8 chips were probed with RNA from 3 to 4 animals per group. Real-time RT-PCR was performed on 6–8 animals per group. Data were analyzed as described in Material and Methods. Average fold change in endometriotic lesions relative to eutopic tissue at each time point is shown. Gene expression was considered altered in endometriotic lesions if the fold change was ≤0.5 or ≥2 and P<.05.
P<.05 for endometriotic lesion vs. eutopic uterus within each time point.
Pelch. Gene expression in mouse endometriosis. Fertil Steril 2010.