Table 1.
Parameters of mucus secretion from individual submucosal glands from ferrets
|
n |
|||||||
|---|---|---|---|---|---|---|---|
| Mean Stable Secretion Rate, pl•min−1•gland−1 | EC50, μM | Latency to Start of Stable Secretion, min | Secretion Rate at Vmax, pl•min−1•gland−1 | Carbachol Vmax Ratio | Ferrets | Glands | |
| Basal | 4.1 ± 0.7 | NA | NA | NA | 0.002* | 27 | 669 |
| VIP (1 μM) | 234 ± 12 | 1.0 | 18 | 479 ± 19 | 0.264 | 6 | 57 |
| Forskolin (10 μM) | 338 ± 70 | 8.7 | 20 | 338 ± 16 | 0.186 | 8 | 90 |
| Carbachol (1 μM) | 978 ± 145 | 0.6 | 2 | 1,817 ± 268 | 1.000 | 11 | 131 |
| Phenylephrine (10 μM) | 1,348 ± 325 | 3.7 | 2 | 1,801 ± 94.7 | 0.991 | 7 | 74 |
| Isoproterenol (10 μM) | 183 ± 92 | ND | 10 | ND | 0.100* | 3 | 33 |
Values are means ± SE. Mean stable secretion rates to the indicated concentrations of agonists are based on as many measurements as possible during the period when secretion had reached a stable value. Vmax ratio is given relative to 10 μM carbachol; Vmax for basal or 10 μM isoproterenol was not determined, so, in those cases, the mean value was used to compute the ratio relative to carbachol-stimulated secretion (indicated with *). NA, not available; ND, not determined.