Table 1.

Oligonucleotides for reporter gene cloning, mutagenesis, EMSA, ChIP assay, and ribonucleotide sequences for siRNA targeting of human AhR

Name	Sequence 5′ → 3′
−2.3kb-KpnI-forward	GGTACCGCTGGGATTATGCGCTTGTAC
−761bp-XhoI-forward	CATACTCTCGAGTTACCCGGCTTTC
−238bp-SmaI-forward	GCTCCCGGGCTCCAGGCGGCGGCGC
−72bp-HindIII-reverse	TCCAAGCTTACCTCAAGCAGGGATGCTG
XRE1 wt	−119GAGCGGACGGCGTGGCGGCCGGAG-
XRE1 mut	−119GAGCGGACGGCTATGCGGCCGGAG−9
XRE2 wt	−181CGGGGACGGGGCGTGCCGCCGGCG−157
XRE2 mut	−181CGGGGACGGGGCTATCCGCCGGCG−157
XRE3 wt	−264GCCGCGCTCGCACGCGGTGCGCAGC−240
XRE3 mut	−264GCCGCGCTCGCAAACGGTGCGCAGC−240
MARE wt	−144AGCGCTGCTTCACAGGCTCC−124
MARE deletion	−144AGCG - -TTCACAGGCTCC−124
Primers for ChIP assay
Forward	CTGCTACCCTCTCCATCCAG
Reverse	ACCTCAAGCCAGGGATGCTG
Sequence of AhR siRNA
1Sense	GUGGAAGUAUUGAUCCAUCUUUCCCU
Antisense	CACCUUCAUAACUAGGUAGAAAGGG
2Sense	AAUGCAACAUCAAAGAAGCUCUUGGCU
Antisense	TTACCGUUGUAGUUUCUUCGAGAACC
3Sense	AUAAAUAGACUCAUCUUGUUGCAUCAU
Antisense	TAUUUAUCUGAGUAGAACAACGUAG

Antisenses were made at the same length and complementary to the sense sequence. ChIP, chromatin immunoprecipitation; siRNA, small interfering RNA; AhR, aryl hydrocarbon receptor; wt, wild type; mut, mutant.