Influence of luminal pH on gastric surface intracellular pH. The gastric epithelium was loaded with SNARF-5F, and pHi was measured before and after photodamage throughout a 20-min time course. From time 0 until 10 min, the tissue was superfused with pH 3 lightly buffered saline and, at 10 min, was switched to either pH 5 (A) or pH 7 (B) lightly buffered saline. C: in other experiments, the tissue was superfused with only pH 7 lightly buffered saline. Data represent means ± SE from 4 experiments per group.