Fig. 4.

Electron micrographs illustrating coimmunogold detection of hPepT1 and glucose transporter 2 (GLUT2) immunogold staining in Caco-2 cells grown on Transwell filters. The micrographs show representative images of brush border (A and B), cytosol (C and D), and basolateral membrane (BLM) (E and F) labeling. Control cells are shown in A, C, and E, and 1 mM AICAR -treated cells are shown in B, D, and F. Arrows indicate immunogold labeling of hPepT1, arrowheads GLUT2. Scale bar represents 200 nM, n, nucleus; f, filter; L, lipid droplet.