Table 1.

Oligonucleotide primer sequences

Oligo Name	Oligo Sequence	Temperature	Start Position	Location	Product Size, bp	Genomic Size, bp	Gene Accession No.
Ngn3_FW	TTTGAGTCGGGAGAACTAGGATGG	57.7°C	220	Exon 1–2	118	0–251	NM_009719.6
Ngn3_RV	TTGGAACTGAGCACTTCGTGGT	58.6°C	337	Exon 2
NeuroD1_FW	AGGAATTCGCCCACGCAGAA	59.3°C	4	Exon 1	101	0–1,603	NM_010894
NeuroD1_RV	TGGTCATGTTTCCACTTCCTGTTGT	58.5°C	104	Exon 2–1
Insulin_FW	TCAAGCAGCACCTTTGTGGT	57.2°C	150		127	0–615	NM_008387.3
Insulin_RV	AGCTCCAGTTGTGCCACTTGT	59.1°C	276
Pax4_FW	AGCTGTGTTGGCTCCAGTTCTT	58.8°C	641	Exon 6	144	0–370	NM_011038.1
Pax4_RV	TGCCCACGCTGAAACTCTTTCT	58.9°C	784	Exon 7–6
Pdx1_FW	CAGTGGGCAGGAGGTGCTTA	59.2°C	508	Exon 1–2	157	3,889	NM_008814.3
Pdx1_RV	AGTTCAACATCACTGCCAGCTC	57.3°C	664	Exon 2
β2-Microglobulin_FW	ACCGGCCTGTATGCTATCCAGAAA		97	Exon 1–2	124		NM_009735.3
β2-Microglobulin_RV	TTTCAATGTGAGGCGGGTGGAA		220	Exon 2
GDNF_FW	TTATGGGATGTCGTGGCTGT	56.3°C	137		618	19,199	NM_010275.2
GDNF_ RV	ACACCGTTTAGCGGAATGCT	57.3°C	754
GAPDH_FW	TTGTGATGGGTGTGAACCACGA	59.0°C			168
GAPDH_RV	TCTTCTGGGTGGCAGTGATGG	59.3°C