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Cellular toxicity of monochloramine as measured using the selectively membrane-permeable dye YO-PRO-1 (●) and the membrane impermeable dye propidium iodide (○). A: glands were incubated with NH2Cl for 1 h, then allowed to recover in Ringer solution for 2 h before staining. B: glands were coincubated with NH2Cl and TPEN (calculated free concentration 20 μM). RFU, relative fluorescence units. *P < 0.05 vs. Ringer control (ANOVA), N = 5, RFUs are expressed as means ± SE.
