Fig. 1.

Effect of resistin on endothelial nitric oxide synthase (eNOS) mRNA levels in human coronary artery endothelial cells (HCAECs). HCAECs were cultured with different concentrations of resistin for different periods of time. The mRNA levels of eNOS and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined by real-time PCR analysis. A: cells were treated with different concentrations of resistin (10, 40, or 80 ng/ml) for 24 h. *P < 0.05, n = 3 experiments. B: cells were treated with resistin (40 ng/ml) for different times (6, 12, and 24 h). *P < 0.05, n = 3. C: cells were treated with 40 ng/ml resistin and different antibodies (Ab) for 24 h. *P < 0.05, n = 3 (compared with untreated controls). #P < 0.05, n = 3 (compared with resistin treatment alone). D: HCAEC eNOS mRNA stability was determined by real-time PCR after cells were treated with 5 μg/ml actinomycin D in the presence or absence of resistin (40 ng/ml) for indicated time points (0, 0.5, 1, 3, 6, or 12 h). *P < 0.05, n = 3 experiments.