Table 2.

Effects on BAT thermogenic and cardiovascular parameters of nanoinjection of PGE₂ into the MPO and of subsequent nanoinjection of Bic into either VLM or NTS

	PGE2 in MPO	Bic in VLM	PGE2 in MPO	Bic in NTS
n	5	5	4	4
BAT SNA, %control	5,850 ± 2,347	298 ± 80*	6,986 ± 1,858	146 ± 43.5*
BAT Temp, °C	35.1 ± 0.5	34.1 ± 0.3*	36.2 ± 0.7	35.3 ± 0.4*
ΔBAT Temp, °C	+1.3 ± 0.4	−1.1 ± 0.2	+1.8 ± 0.4	−0.8 ± 0.4
Exp CO2, %	4.6 ± 0.3	4.2 ± 0.3*	5.0 ± 0.3	4.7 ± 0.2*
ΔExp CO2, %	+0.8 ± 0.2	−0.4 ± 0.1	+1.1 ± 0.2	−0.4 ± 0.1
HR, beats/min	494 ± 16	458 ± 36	495 ± 15	422 ± 46
ΔHR, beats/min	+63 ± 20	−35 ± 22	+84 ± 21	−73 ± 34
MAP, mmHg	103 ± 4.8	163 ± 5.6†	117 ± 5.9	105 ± 9.0*
ΔMAP, mmHg	+12 ± 4.7	+59 ± 3.3	+20 ± 4.1	−12 ± 3.9
RSNA, %control	151 ± 18.5	211 ± 30*	132 ± 13.1	103 ± 15.9*

Values are means ± SE; n, no. of rats. PGE₂, prostaglandin E₂; MPO, medial preoptic area; NTS, nucleus tractus solitarius; RSNA, renal SNA. Mean parameter values for “PGE₂ in MPO” are the group means for the parameter values during the 1-min interval before the subsequent Bic nanoinjections into either VLM or NTS, and the corresponding changes for “PGE₂ in MPO” are relative to the mean parameter values during the 1 min before nanoinjection of PGE₂ into the MPO. Mean parameter values for “Bic in VLM” and “Bic in NTS” are the group means for the parameter values during the 5th min (or 8th min for BAT Temp only) following the Bic nanoinjections. The values for BAT SNA and RSNA are expressed as percentage of pre-“PGE₂ in MPO” control levels. For other parameters, the changes for “Bic in VLM” or “Bic in NTS” are relative to the mean “PGE₂ in MPO” parameter values (i.e., during the 1 min before nanoinjection of Bic into VLM or NTS). Core (rectal) temperatures were maintained between 36.5 and 37.5°C with a servo-controlled heating lamp. Statistically significant compared with control value following saline into VLM or NTS:

^*P< 0.05,

^†P< 0.01 (paired t-test).