Table 3.

Effects on BAT thermogenic and cardiovascular parameters of nanoinjection of Bic into the DMH and of subsequent nanoinjection of Bic into either VLM or NTS

	Bic in DMH	Bic in VLM	Bic in DMH	Bic in NTS
n	6	6	7	7
BAT SNA, %control	5,097 ± 1,131	184 ± 16*	6,016 ± 1,272	121 ± 28.3†
BAT Temp, °C	36.1 ± 0.3	35.6 ± 0.3	35.8 ± 0.5	35.0 ± 0.4
ΔBAT Temp, °C	+0.8 ± 0.2	−0.5 ± 0.1*	+1.3 ± 0.2	−0.7 ± 0.2†
Exp CO2, %	4.2 ± 0.1	3.8 ± 0.0	4.4 ± 0.1	4.2 ± 0.1
ΔExp CO2, %	+0.4 ± 0.1	−0.4 ± 0.1*	+0.5 ± 0.1	−0.2 ± 0.1†
HR, beats/min	465 ± 16	458 ± 18	467 ± 13	357 ± 29
ΔHR, beats/min	+70 ± 8	−7 ± 15	+68 ± 14	−110 ± 28†
MAP, mmHg	131 ± 4.5	186 ± 6.7	128 ± 5.4	98 ± 7.3
ΔMAP, mmHg	+24 ± 10.2	+55 ± 5.3*	+16 ± 2.5	−30 ± 2.6†
RSNA, %control	161 ± 15.6	242 ± 36*	175 ± 11.2	94 ± 3.9†

Values are means ± SE; n, no. of rats. DMH, dorsomedial hypothalamus. Mean parameter values for “Bic in DMH” are the group means for the parameter values during the 1-min interval before the subsequent Bic nanoinjections into either VLM or NTS, and the corresponding changes for “Bic in DMH” are relative to the mean parameter values during the 1 min before nanoinjection of Bic into the DMH. Mean parameter values for “Bic in VLM” and “Bic in NTS” are the group means for the parameter values during the 5th min (or 8th min for BAT Temp only) following nanoinjections of Bic into the VLM or NTS. The values for BAT SNA and RSNA are expressed as percentage of pre-“Bic in DMH” control levels. For other parameters, the changes for “Bic in VLM” or “Bic in NTS” are relative to the mean “Bic in DMH” parameter values (i.e., during the 1 min before nanoinjections of Bic into VLM or NTS). Core (rectal) temperatures were maintained between 36.5 and 37.5°C with a servo-controlled heating lamp. Statistically significant compared with control value following saline into VLM or NTS:

^*P< 0.05;

^†P< 0.01 (paired t-test).