Table 4.

Effects on BAT thermogenic and cardiovascular parameters of nanoinjection of Bic in rRPa and of subsequent disinhibition of neurons in either VLM or NTS

	Bic in RPa	Bic in VLM	Bic in RPa	Bic in NTS
n	6	6	6	6
BAT SNA, %control	4,919 ± 826	364 ± 33†	6,993 ± 1,478	189 ± 26†
BAT Temp, °C	35.7 ± 0.4	35.1 ± 0.4	35.5 ± 0.4	34.7 ± 0.4
ΔBAT Temp, °C	+1.1 ± 0.2	−0.6 ± 0.2*	+1.3 ± 0.2	−0.8 ± 0.2†
Exp CO2, %	4.2 ± 0.1	4.1 ± 0.1	4.6 ± 0.1	4.4 ± 0.1
ΔExp CO2, %	+0.3 ± 0.0	−0.1 ± 0.1*	+0.5 ± 0.1	−0.2 ± 0.0†
HR, beats/min	483 ± 19	455 ± 17	463 ± 14	359 ± 30
ΔHR, beats/min	+51 ± 11	−80 ± 22*	+28 ± 10	−104 ± 21†
MAP, mmHg	97 ± 6.7	161 ± 9.2	109 ± 10.8	102 ± 9.8
ΔMAP, mmHg	+10 ± 3.2	+64 ± 9.1†	+7 ± 2.6	−7 ± 2.8*

Values are means ± SE; n, no. of rats. rRPa, rostral raphe pallidus. Mean parameter values for “Bic in rRPa” are group means of the parameter values during the 1-min interval before the subsequent Bic nanoinjections into either VLM or NTS, and the corresponding changes for “Bic in rRPa” are relative to the mean parameter values during the 1 min before nanoinjection of “Bic into the rRPa”. Mean parameter values for “Bic in VLM” or “Bic in NTS” are group means of the parameter values during the 5th min (or 8th min for BAT Temp only) following nanoinjections of Bic into the VLM or NTS. The values for BAT SNA are expressed as percentage of pre-“Bic in rRPa” control levels. For other parameters, the changes for “Bic in VLM” or “Bic in NTS” are relative to the mean “Bic in rRPa” parameter values (i.e., during the 1 min before nanoinjections of Bic into VLM or NTS). Core (rectal) temperatures were maintained between 36.5 and 37.5°C with a servo-controlled heating lamp. Statistically significant compared with control value following saline into VLM or NTS:

^*P< 0.05;

^†P< 0.01 (paired t-test).