Table 6.

Effects on sympathetic BAT thermogenic and cardiovascular parameters of combined inhibition of neurons in rostral VLM and in NTS, of subsequent disinhibition of neurons in rRPa and of subsequent inhibition of neurons in rRPa

	Control	Musc in NTS and VLM	Bic in rRPa	Gly in rRPa
n	14	14	8	11
BAT SNA, %control	100	4,001 ± 1,035†	12,090 ± 2,645‡	67 ± 14§
BAT Temp, °C	33.6 ± 0.2	34.0 ± 0.3†	34.6 ± 0.3‡	33.8 ± 0.2§
ΔBAT Temp, °C		+0.5 ± 0.1	+0.8 ± 0.1	−1.0 ± 0.2
Exp CO2, %	4.0 ± 0.1	3.7 ± 0.2†	4.0 ± 0.2‡	3.6 ± 0.2§
ΔExp CO2, %		−0.3 ± 0.1	+0.3 ± 0.1	−0.4 ± 0.1
HR, beats/min	463 ± 8	400 ± 18†	463 ± 12‡	375 ± 21§
ΔHR, beats/min		−64 ± 13	+61 ± 16	−94 ± 17
MAP, mmHg	80 ± 6	46 ± 3†	44 ± 4	37 ± 3§
ΔMAP, mmHg		−35 ± 5	+1 ± 1	−5 ± 1
RSNA, %control		54 ± 5.8†	53 ± 5.8	48 ± 3.7

Values are means ± SE; n, no. of rats. Musc, muscimol; Gly, glycine. The values for BAT SNA and RSNA are expressed as percentage of pre-“Musc in NTS and VLM” control levels. Mean parameter values for “Musc in NTS and VLM” are the group means of the parameter values during the 1-min interval representing the peak response within 15 min of the final Musc nanoinjection, and the corresponding changes for “Musc in NTS and VLM” are relative to the mean parameter values during the 1 min before the first nanoinjection of Musc into NTS. Mean parameter values for “Bic in rRPa” and for “Gly in rRPa” are the group means of the parameter values during the 1-min interval representing the peak response within 5 min of the nanoinjection of Bic into rRPa and of Gly into rRPa, respectively, and the corresponding changes for “Bic in rRPa” and for “Gly into rRPa” are relative to the “Musc in NTS and VLM” and “Bic in rRPa” parameter values, respectively. Core (rectal) temperatures were maintained between 36.5 and 37.5°C with a servo-controlled heating lamp. Statistically significant compared with control value following saline into NTS and VLM:

^†P< 0.01 (paired t-test); from “Musc in NTS and VLM” value:

^‡P< 0.01; and from “Bic in rRPa” value:

^§P< 0.01.