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Dephosphorylation of Drp1 at serine-637 during ATP depletion. Rat kidney proximal tubular cell line (RPTC) cells were subjected to 0–3 h of ATP depletion with 10 mM azide in glucose-free buffer. One group of the cells was returned to full culture medium for 2 h of recovery. Whole cell lysates were collected for immunoblot analysis of phosphorylated (serine-637) Drp1, total Drp1, and β-actin. The blots are representative of 3 experiments.
