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. 2010 Apr 14;299(1):F187–F198. doi: 10.1152/ajprenal.00607.2009

Fig. 3.

Fig. 3.

Expression of Rhbg in the developing kidney. A: at embryonic day 16 (E16), detectable Rhbg immunolabel was not expressed in developing glomeruli (G), ureteric bud (UB), or interstitium. B–D: Rhbg expression at E18. B: low-power micrograph of Rhbg immunolabel in the E18 rat kidney. Expression is present in a subset of convoluted tubule cells in the cortex, primarily in the corticomedullary junction region. C: high-power micrograph of cortex showing basolateral Rhbg expression in a subset of connecting segment cells. D: high-power micrograph of medullary collecting duct (MCD) demonstrating that specific Rhbg immunolabel is not present at E18. E–G: Rhbg in the E20 kidney. Rhbg expression in the cortex is predominantly in the inner cortical region in CNT segments and is increased relative to E18. Medullary Rhbg expression is now present and is greatest in the distal medulla. F: high-power micrograph demonstrating basolateral Rhbg immunolabel in a subset of connecting segment cells (arrow). G: simultaneous intense basolateral Rhbg immunolabel in a subset of MCD cells. The distinct basolateral Rhbg expression present in many, but not all, MCD cells is consistent with differential expression in intercalated cells and principal cells. H: low-power micrograph at postnatal day 1 (P1). Rhbg is expressed at increasing levels in connecting segments in the cortex and in collecting duct segments in the medulla. CCD segments do not express significant Rhbg immunolabel. In the medulla, Rhbg expression is greatest in the distal MCD, with relatively less expression in the MCD adjacent to the corticomedullary junction. I: high-power micrograph of the medulla of the P7 kidney. There is the beginning of the disappearance of Rhbg immunolabel from the distal MCD and increased expression in a subset of cells in the MCD closer to the corticomedullary junction. J: Rhbg expression in the P14 kidney. There is a further increase in Rhbg immunolabel in the cortex, almost complete absence of Rhbg-expressing cells in the distal MCD, and increased expression in more proximal regions of the MCD.