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Knockdown of Dot1a and AF9 upregulates mRNA and protein expression of ENaC and Sgk1. A–C: IMCD3 cells stably transfected with an empty vector (Vec), pDot1aRNAi2 (siDot1a) (48), or pAF9RNAi2 (siAF9) (49) were examined by real-time RT-PCR (A and B) or immunoblotting (C) for expression of the genes indicated as in Fig. 4; n = 3. *P < 0.05 vs. vector for each gene.
