Fig. 5.

Glycated albumin treatment increased nuclear NF-κB subunit-p65 protein levels. Quiescent RMCs were treated with glycated or control albumin (200 μg/ml) for 24 h. After treatment, cells were harvested and nuclear extracts were prepared. The protein levels of NF-κB subunits-p65 (A) and p50 (B) in the nuclear extracts were analyzed by immunoblotting. TATA-binding protein (TBP) was used as an internal control. The blots shown are representative of 3 separate experiments. Relative p65 or p50 levels were determined by scanning densitometry of blots. Data are expressed as means ± SE of 3 separate experiments. *P < 0.05 vs. control albumin.