Fig. 8.

AMPK regulation of CRT may involve the mammalian target of rapamycin (mTOR) pathway. Xenopus oocytes were injected with CRT and mTOR cRNAs 5–6 days before 2-electrode voltage-clamp (TEV) measurements and treated with DMSO (vehicle) or 50 nM rapamycin for 3 days before TEV measurements. Oocytes from control and rapamycin-treated groups were then injected with K-ZMP (ZMP) or K-gluconate (KG) 5–8 h before TEV measurements. CRT-dependent current decreased significantly in mTOR-expressing oocytes treated with ZMP compared with control oocytes treated with KG (left). ZMP did not have a significant effect in oocytes expressing mTOR and treated with rapamycin (right). *P < 0.05 and #P < 0.05 vs. mTOR-alone control (n = 17–21 oocytes per condition, N = 3 batches).