Table 2.
Summary of key findings: association between miRNA and gene expression in response to brief exercise in human neutrophils and gene pathways that were significantly affected
| miRNA and Fold Change | Target Genes (no.): Enrich in Kegg Pathways EASE < 0.05 | Genes Affected by Exercise 1) Known To Be Targeted by the Specific miRNA; and 2) Whose Expression Level Was Altered by Exercise in Our Previous Study | Common Genes Enrich in Kegg Pathways EASE ≤ 0.05 |
|---|---|---|---|
| hsa-miR-20a ↓1.24 | 870 Target genes:TGF-β signaling pathway, Prostate cancer, Renal cell carcinoma, Axon guidance, Glioma, Bladder cancer, Chronic myeloid leukemia, Melanoma, Pancreatic cancer, Non-small cell lung cancer, Colorectal cancer, ErbB signaling pathway, MAPK signaling pathway, Endometrial cancer, Regulation of actin cytoskeleton, mTOR signaling pathway, Acute myeloid leukemia, Small cell lung cancer, Focal adhesion, Circadian rhythm, Adherens junction | 36 Genes: ANKRD12(↓), HBP1(↓), C10orf46(↓), DNAJB9(↓), PTEN(↓), SSH2(↓), DNAJB9(↓), UBE2J1(↓), STK38(↑), TAGAP(↑), USP24(↑), NPAT(↑), ZNF148(↑), SERTAD2(↑), FBXW11(↑), CNOT6L(↑), TRIM37(↑), ATP2B4(↑), CENTD1(↑), SYNE1(↑), PIK3R1(↑), SLC5A3(↑), TMEM50B(↑), MAP3K12(↑), ARHGEF3(↑), C1orf63(↑), PIK3R1(↑), AHNAK(↑), HSPA8(↑), SMAD7(↑), CCND2(↑), PAFAH2(↑), PTPN4(↑), RUNX3(↑), DUSP2(↑), LDLR(↑) | Ubiquitin-mediated proteolysis |
| hsa-miR-106a ↓1.39 | 880 Target genes: Axon guidance, TGF-β signaling pathway, Bladder cancer, Prostate cancer, Renal cell carcinoma, Glioma, Chronic myeloid leukemia, MAPK signaling pathway, Melanoma, Pancreatic cancer, Non-small cell lung cancer, Colorectal cancer, ErbB signaling pathway, Circadian rhythm, Endometrial cancer, mTOR signaling pathway, Regulation of actin cytoskeleton, Acute myeloid leukemia, Small cell lung cancer, Adherens junction | 35 Genes: ANKRD12(↓), HBP1(↓), C10orf46(↓), SRGAP2(↓), PTEN(↓), SSH2(↓), DNAJB9(↓), UBE2J1(↓), STK38(↑), TAGAP(↑), USP24(↑), NPAT(↑), ZNF148(↑), SERTAD2(↑), FBXW11(↑), CNOT6L(↑), TRIM37(↑), ATP2B4(↑), CENTD1(↑), SYNE1(↑), SLC5A3(↑), TMEM50B(↑), MAP3K12(↑), ARHGEF3(↑), C1orf63(↑), KLF12(↑), AHNAK(↑), HSPA8(↑), SMAD7(↑), CCND2(↑), PAFAH2(↑), PTPN4(↑), RUNX3(↑), DUSP2(↑), LDLR(↑) | Ubiquitin-mediated proteolysis |
| hsa-miR-20b ↓1.29 | 871 Target genes: TGF-β signaling pathway, Axon guidance, Bladder cancer, Prostate cancer, Renal cell carcinoma, Glioma, Chronic myeloid leukemia, Melanoma, Pancreatic cancer, MAPK signaling pathway, Nonsmall cell lung cancer, Colorectal cancer, ErbB signaling pathway, Regulation of actin cytoskeleton, Circadian rhythm, Endometrial cancer, mTOR signaling pathway, Focal adhesion, Acute myeloid leukemia, Small cell lung cancer, Adherens junction | 34 Genes: ANKRD12(↓), HBP1(↓), C10orf46(↓), PTEN(↓), SSH2(↓), DNAJB9(↓),UBE2J1(↓), STK38(↑), TAGAP(↑), USP24(↑), NPAT(↑), ZNF148(↑), SERTAD2(↑), FBXW11(↑), CNOT6L(↑), TRIM37(↑), ATP2B4(↑), CENTD1(↑), SYNE1(↑), SLC5A3(↑), TMEM50B(↑), MAP3K12(↑), ARHGEF3(↑), C1orf63(↑), KLF12(↑), AHNAK(↑), HSPA8(↑), SMAD7(↑), CCND2(↑), PAFAH2(↑), PTPN4(↑), RUNX3(↑), DUSP2(↑), LDLR(↑) | Ubiquitin-mediated proteolysis |
| hsa-miR-17 ↓1.40 | 875 Target genes: TGF-β signaling pathway, Axon guidance, Bladder cancer, Prostate cancer, Renal cell carcinoma, Glioma, Chronic myeloid leukemia, MAPK signaling pathway, Melanoma, Pancreatic cancer, Non-small cell lung cancer, Colorectal cancer, ErbB signaling pathway, Circadian rhythm, Endometrial cancer, mTOR signaling pathway, Regulation of actin cytoskeleton, Acute myeloid leukemia, Small cell lung cancer, Adherens junction | 37 Genes: ANKRD12(↓), HBP1(↓), C10orf46(↓), PTEN(↓), SSH2(↓), DNAJB9(↓),UBE2J1(↓), STK38(↑), TAGAP(↑), TAGAP(↑), USP24(↑), NPAT(↑), ZNF148(↑), SERTAD2(↑), FBXW11(↑), CNOT6L(↑), TRIM37(↑), ATP2B4(↑), CENTD1(↑), TAGAP(↑), SYNE1(↑), SLC5A3(↑), TMEM50B(↑), MAP3K12(↑), TAGAP(↑), ARHGEF3(↑), C1orf63(↑), KLF12(↑), AHNAK(↑), HSPA8(↑), SMAD7(↑), CCND2(↑), PAFAH2(↑), PTPN4(↑), RUNX3(↑), DUSP2(↑), LDLR(↑) | Ubiquitin-mediated proteolysis |
| hsa-miR-93 ↓1.239805 | 871 Target genes: TGF-β signaling pathway, Axon guidance, Bladder cancer, Prostate cancer, Renal cell carcinoma, Glioma, Chronic myeloid leukemia, MAPK signaling pathway, Melanoma, Pancreatic cancer, Regulation of actin cytoskeleton, Nonsmall cell lung cancer, ErbB signaling pathway, Adherens junction, Circadian rhythm, Endometrial cancer, mTOR signaling pathway, Acute myeloid leukemia, Colorectal cancer Small cell lung cancer | 31 Genes: ANKRD12(↓), HBP1(↓), PTEN(↓), SSH2(↓), DNAJB9(↓), UBE2J1(↓), STK38(↑), TAGAP(↑), USP24(↑), NPAT(↑), ZNF148(↑), SERTAD2(↑), FBXW11(↑), CNOT6L(↑), TRIM37(↑), CENTD1(↑), SYNE1(↑), SLC5A3(↑), TMEM50B(↑), MAP3K12(↑), ARHGEF3(↑), C1orf63(↑), AHNAK(↑), HSPA8(↑), SMAD7(↑), CCND2(↑), PAFAH2(↑), PTPN4(↑), RUNX3(↑), DUSP2(↑), LDLR(↑) | Ubiquitin-mediated proteolysis |
| hsa-miR-520d-3p ↑2.82 | 515 Target genes: Colorectal cancer, mTOR signaling pathway, MAPK signaling pathway, ECM-receptor interaction, Focal adhesion, TGF-β signaling pathway, Regulation of actin cytoskeleton, Pancreatic cancer, Endometrial cancer | 19 Genes: TARDBP(↓), UBE2B(↓), UBE2J1(↓), WDR68(↑), TAGAP(↑), USP24(↑), ZNF148(↑), ATP2B4(↑), RALGDS(↑), TIPARP(↑), ARHGEF3(↑), UBE3A(↑), ASF1A(↑), PRKACB(↑), AHNAK(↑), TOX(↑), CCND2(↑), DUSP2(↑), MYBL1(↑) | Ubiquitin mediated proteolysis |
| hsa-miR-130b ↓1.41 | 599 Target genes: TGF-β signaling pathway, mTOR signaling pathway, Colorectal cancer, Renal cell carcinoma, Prostate cancer, Pancreatic cancer, Chronic myeloid leukemia, Hedgehog signaling pathway, Glioma,mErbB signaling pathway, Wnt signaling pathway, Axon guidance, MelanoTarget genesis, Focal adhesion, Ubiquitin-mediated proteolysis | 27 Genes: ANKRD12(↓), TARDBP(↓), HBP1(↓), KIF13A(↓), ARFGEF1(↓), DICER1(↓), SBF2(↓), BAG5(↓), NPAT(↑), PHF20(↑), P15RS(↑), ZNF148(↑), CBFB(↑), FBXW11(↑), TRIM37(↑), CENTD1(↑), TMEM50B(↑), MAP3K12(↑), PHACTR2(↑), PRKACB(↑), HSPA8(↑), PTPN4(↑), RUNX3(↑), MAF(↑), LDLR(↑), ENPP4(↑), MYBL1(↑) | Hedgehog signaling pathway |
| hsa-miR-16 ↓1.23 | 776 Target Genes: Acute myeloid leukemia, Insulin signaling pathway, Prostate cancer, Cell cycle, Chronic myeloid leukemia, Non-small cell lung cancer, Ubiquitin-mediated proteolysis, Endometrial cancer, Melanoma, Colorectal cancer, Pancreatic cancer, Glioma, Adherens junction, TGF-β signaling pathway, mTOR signaling pathway, Renal cell carcinoma, Wnt signaling pathway, Thyroid cancer, Hedgehog signaling pathway, Type II diabetes mellitus, MAPK signaling pathway, Gap junction, O-glycan biosynthesis | 30 Genes: ZCCHC2(↓), C10orf46(↓), DICER1(↓), UBE2J1(↓), BAG5(↓), AP1GBP1(↑), TUBB(↑), WDR68(↑), PHF20(↑),OTUD4(↑), SLC2A3(↑), C13orf18(↑), MGAT4A(↑), CNOT6L(↑), TRIM37(↑), PURA(↑), CDC42EP2(↑), PIK3R1(↑), SLC5A3(↑), PHACTR2(↑), IL10RA(↑), SMAD3(↑), PLEKHA1(↑), SMAD7(↑), CCND2(↑), RNF125(↑), SH2D1A(↑), SYTL2(↑), TGFBR3(↑), MYBL1(↑) | Jak-STAT signaling pathway |
| hsa-let-7i ↓1.25 | 558 Target genes: p53 signaling pathway, ECM-receptor interaction, MAPK signaling pathway, Colorectal cancer, Pancreatic cancer, Focal adhesion, O-glycan biosynthesis, mTOR signaling pathway, Melanoma, TGF-β signaling pathway, Chronic myeloid leukemia, Adherens junction | 14 Genes: ZNF294(↓), RIOK3(↓), PMAIP1(↓), KLHL6(↓), USP24(↑), MGAT4A(↑), TMEM2(↑), DNAJC1(↑), ZFP36L2(↑), SLAMF6(↑), PHACTR2(↑), RASGRP1(↑), GNPTAB(↑), CREM(↑) | None |
| hsa-miR-107 ↓1.26 | 376 Target genes: Hedgehog signaling pathway, O-glycan biosynthesis, Ubiquitin-mediated proteolysis, Basal cell carcinoma, Circadian rhythm, Leukocyte transendothelial migration, Wnt signaling pathway, MelanoTarget genesis | 17 Genes: ZCCHC2(↓), EIF5(↓), DICER1(↓), UBE2J1(↓), PHF20(↑), OTUD4(↑), SMEK2(↑), CUL4A(↑), ENSA(↑), CNOT6L(↑), PIK3R1(↑), SLC5A3(↑), PLEKHA1(↑), RNF125(↑), EOMES(↑), TGFBR3(↑), MYBL1(↑) | None |
| hsa-miR-126 ↓1.53 | 12 Target genes: NO Kegg pathway | None | None |
| hsa-miR-130a ↓1.61 | 601 Target genes: TGF-β signaling pathway, mTOR signaling pathway, Renal cell carcinoma, Colorectal cancer, ErbB signaling pathway, Prostate cancer, Hedgehog signaling pathway, Glioma, Dorso-ventral axis formation, MelanoTarget genesis, Wnt signaling pathway, Axon guidance, Pancreatic cancer, Chronic myeloid leukemia, Focal adhesion | 28 Genes: ANKRD12(↓), TARDBP(↓), HBP1(↓), KIF13A(↓), ARFGEF1(↓), DICER1(↓), SBF2(↓), BAG5(↓), NPAT(↑), PHF20(↑), P15RS(↑), ZNF148(↑), CBFB(↑), FBXW11(↑), TRIM37(↑), ATP2B4(↑), CENTD1(↑), TMEM50B(↑), MAP3K12(↑), PHACTR2(↑), PRKACB(↑), HSPA8(↑), PTPN4(↑), RUNX3(↑), MAF(↑), LDLR(↑), ENPP4(↑), MYBL1(↑) | None |
| hsa-miR-151-5p ↓1.60 | 5 Target genes: NO Kegg pathway | None | None |
| hsa-miR-185 ↓1.28 | 134 Target genes: GnRH signaling pathway | 1 Gene: MAP4(↑) | None |
| hsa-miR-18a ↓1.35 | 169 Target genes: Tight junction, p53 signaling pathway | 7 Genes: DICER1(↓), USP24(↑), TMEM2(↑), LMO4(↑), CCND2(↑), ENC1(↑), PDE4D(↑) | None |
| hsa-miR-18b ↓1.29 | 169 Target genes: p53 signaling pathway, Tight junction | 7 Genes: DICER1(↓), USP24(↑), TMEM2(↑), LMO4(↑), CCND2(↑), ENC1(↑), PDE4D(↑) | None |
| hsa-miR-194 ↓1.26 | 185 Target genes: TGF-β signaling pathway, ErbB signaling pathway | 3 Genes: MGAT4A(↑), PDE4D(↑), SOCS2(↑) | None |
| hsa-miR-22 ↓1.28 | 276 Target genes: Chronic myeloid leukemia, Adherens junction, ErbB signaling pathway, Pancreatic cancer, Alkaloid biosynthesis II | 8 Genes: H3F3B(↓), PTEN(↓), MAX(↓), CNOT6L(↑), TMEM50B(↑), PHACTR2(↑), DDIT4(↑), SOCS2(↑) | None |
| hsa-miR-363 ↓1.34 | 555 Target genes: Regulation of actin cytoskeleton, Calcium signaling pathway, Adherens junction, Long-term potentiation, Focal adhesion | 21 Genes: H3F3B(↓), ING3(↓), PTEN(↓), PTEN(↓), HIVEP1(↓), DNAJB9(↓), NFYC(↓), C10orf118(↓), TAGAP(↑), OTUD4(↑), TMF1(↑), FOSL2(↑), ATP2B4(↑), PTGER4(↑), TMEM50B(↑), PLEKHA1(↑), DDIT4(↑), SMAD7(↑), NPC1(↑), NPC1(↑), EOMES(↑) | None |
| hsa-miR-660 ↓1.231584 | 67 Target genes: Regulation of actin cytoskeleton, GnRH signaling pathway | 1 Gene: TOX(↑) | None |
| hsa-miR-96 ↓1.28 | 578 Target genes: Long-term potentiation, GnRH signaling pathway, Gap junction, ErbB signaling pathway, Long-term depression, Glioma, Tight junction, Focal adhesion, Prostate cancer, MAPK signaling pathway, Fc epsilon RI signaling pathway, Calcium signaling pathway, Glycosphingolipid biosynthesis - ganglioseries, Renal cell carcinoma, Natural killer cell-mediated cytotoxicity, Axon guidance, Insulin signaling pathway, Chronic myeloid leukemia, T cell receptor signaling pathway, Phosphatidylinositol signaling system, MelanoTarget genesis, Regulation of actin cytoskeleton | 13 Genes: ELAVL1(↑), FBXW11(↑), CNOT6L(↑), FYN(↑), SMAD7(↑), ATP2B4(↑), ARHGEF3(↑), PIK3R1(↑), UBE2L3(↑), TMEM50B(↑), EOMES(↑), NCALD(↑), TOX(↑) | None |
| hsa-miR-1225-5p ↑1.55 | 41 Target genes: NO Kegg pathway | None | |
| hsa-miR-1238 ↑1.58 | 61 Target genes: TGF-β signaling pathway | 2 Genes: SLC8A1(↓), NCALD(↑) | None |
| hsa-miR-125a-5p ↑1.22 | 506 Target genes: TGF-β signaling pathway, Glycerolipid metabolism, O-glycan biosynthesis | 13 Genes: KLHL6(↓), DICER1(↓), IER2(↓), UBE2J1(↓), PHF23(↑), PHF20(↑), ZNF148(↑), MGAT4A(↑), UBE2L3(↑), TSEN54(↑), MAPRE2(↑), SLC7A1(↑), MAF(↑) | None |
| hsa-miR-145 ↑1.22 | 439 Target genes: Axon guidance, TGF- β signaling pathway, Chronic myeloid leukemia, Wnt signaling pathway, Adherens junction | 13 Genes: AP1G1(↓), C7orf28B(↓), SRGAP2(↓), CCNL1(↓), SSH2(↓), CHP(↓), CENTD1(↑), H2AFX(↑), PHACTR2(↑), SMAD3(↑), SLC7A1(↑), RUNX3(↑), PDGFD(↑) | None |
| hsa-miR-181b ↑1.64 | 652 Target genes: Dorso-ventral axis formation, Axon guidance, Long-term potentiation, Renal cell carcinoma, TGF-β signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway | 24 Genes: AP1G1(↓), TARDBP(↓), SF1(↓), BCLAF1(↓), SRGAP2(↓), UBE2B(↓), UBE2J1(↓), KLF6(↑), OTUD4(↑), SLC2A3(↑), TMF1(↑), HYOU1(↑), ATP2B4(↑),RALA(↑), PHACTR2(↑), ARHGEF3(↑), SLC38A2(↑), DMXL2(↑), DDIT4(↑), TOX(↑), SMAD7(↑), RNF125(↑), NCALD(↑), MYBL1(↑) | None |
| hsa-miR-193a-3p ↑1.58 | 120 Target genes: Acute myeloid leukemia, Dorso-ventral axis formation, Endometrial cancer, Renal cell carcinoma, Thyroid cancer, Colorectal cancer, Prostate cancer, Focal adhesion, Non-small cell lung cancer | 2 Genes: DNAJB9(↓), WDR68(↑) | None |
| hsa-miR-197 ↑1.38 | 117 Target genes: Ubiquitin-mediated proteolysis | 2 Genes: SSH2(↓), SMEK2(↑) | None |
| hsa-miR-212 ↑1.44 | 219 Target genes: Prostate cancer, TGF-β signaling pathway, Axon guidance, Endometrial cancer, GnRH signaling pathway, Renal cell carcinoma, MelanoTarget genesis, Adherens junction, Non-small cell lung cancer, Colorectal cancer, ErbB signaling pathway, Thyroid cancer | 8 Genes: EGR1(↓), SRGAP2(↓), H3F3B(↓), SSH2(↓), FBXW11(↑), TMEM2(↑), FLJ10357(↑), NCALD(↑) | None |
| hsa-miR-223 ↑1.29 | 180 Target genes: NO Kegg pathway | 8 Genes: AP1GBP1(↑), RHOB(↑), PDE4D(↑), PRKACB(↑), PURA(↑), RGS1(↑), SLC8A1(↓), C13orf18(↑) | None |
| hsa-miR-340* ↑1.25 | No target genes | None | |
| hsa-miR-365 ↑1.37 | 139 Target genes: Wnt signaling pathway, MelanoTarget genesis, Apoptosis, Prostate cancer | 6 Genes: CHP(↓), SSH2(↓), CNOT6L(↑), HSPA8(↑), ZNF148(↑), SERTAD2(↑) | None |
| hsa-miR-485-3p ↑2.92 | 240 Target genes: Wnt signaling pathway, Adherens junction, MAPK signaling pathway, VEGF signaling pathway, Long-term potentiation, Pancreatic cancer, Chronic myeloid leukemia, Colorectal cancer | 5 Genes: MGAT4A(↑), WDR7(↑), PDE4D(↑), OTUD4(↑), SLC5A3(↑) | None |
| hsa-miR-505 ↑1.24 | 130 Target genes: Tight junction, Cell cycle | 2 Genes: H3F3B(↓), SF1(↓) | None |
| hsa-miR-629* ↑1.44 | No target genes | None | |
| hsa-miR-638 ↑1.36 | 17 Target genes: NO Kegg pathway | 2 Genes: PGK1(↓), LMO4(↑) | None |
| hsa-miR-939 ↑1.40 | 75 Target genes: NO Kegg pathway | 2 Genes:TSC22D3(↑), LDOC1L(↑) | None |
| hsa-miR-940 ↑1.39 | 203 Target genes: T cell receptor signaling pathway, MAPK signaling pathway, Axon guidance, Focal adhesion | 4 Genes: SSH2(↓), CHP(↓), CNOT6L(↑), PDE4D(↑) | None |