Figure 2.

Activation of MAPK pathway in the mammary tumors of H-Ras transgenic mice. (A) Protein extracted from normal (N) and mammary tumor tissues (T) of Cdk4(neo/neo):MMTV-v-Ha-ras and Cdk4(+/+):MMTV-v-Ha-ras mice was analyzed for Ras, Phospho-Raf1 (P-Raf1), Raf-1, Phospho-MEK1/2 (P-MEK1/2), and MEK1/2 by Western blot analysis. (B) Formalin-fixed paraffin-embedded normal and tumor sections of the mammary glands of Cdk4(+/+):MMTV-v-Ha-ras mice were subjected to immunohistochemical analysis using antibody against Ras (40x). (C) Presence of increased levels of cell cycle proteins in Ras-induced breast tumors. Protein extracted from normal (N) and mammary tumor tissues (T) of Cdk4(neo/neo):MMTV-v-Ha-ras and Cdk4(+/+):MMTV-v-Ha-ras mice was analyzed for Cdk4, Cdk6, Cdk2, and P-pRb (Ser780).

Note: Trans. = Transgene; n/n = neo/neo.