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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: J Cell Sci. 2008 Nov 11;121(Pt 23):3981–3990. doi: 10.1242/jcs.033431

Figure 6. RUNX1/AML1 associates with hypermethylated rDNA repeats.

Figure 6

Chromatin from Kasumi cells was immunoprecipitated with antibodies against RUNX1/AML1 or AML1-ETO and the resulting DNA was digested with McrBC enzyme prior to qPCR using indicated rDNA primers. An antibody detecting ETO was used to immunoprecipitate the AML1-ETO fusion protein, whereas an antibody recognizing the C-terminal domain of RUNX1/AML1 was used to pull down endogenous RUNX1/AML1. Quantitative PCR data are normalized to genomic DNA and denoted as percent input. These results indicate that rDNA repeats bound by RUNX1/AML1 are hyper-methylated relative to those that are bound by AML1-ETO.