Skip to main content
. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: J Cell Sci. 2008 Nov 11;121(Pt 23):3981–3990. doi: 10.1242/jcs.033431

Figure 8. RUNX1/AML1 or AML1-ETO deficiency alters rRNA synthesis.

Figure 8

Kasumi-1 cells were transfected with two independent RUNX1/AML1 or AML1-ETO siRNAs or non-silencing (NS) siRNA. A) To check the efficiency of knockdown, protein expression of RUNX1/AML1, AML1-ETO and α-tubulin was examined by western blot analysis. B) Expression of unprocessed rRNA (pre-rRNA synthesis), 28s RNA and p21 was examined by RT-PCR analysis. Bars represent expression levels relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA (±SEM) from three independent experiments performed in triplicate. C) Epitope tagged RUNX1/AML1 and AML1-ETO were each ectopically expressed in mouse 32D myeloid progenitor cells. Pre-rRNA synthesis was measured by RT-qPCR relative to GAPDH in equal numbers of cells (top). Expression of the exogenous proteins was analyzed by western blot analysis (bottom).